521-321 Gene Technology & Protein Expression

Before the commencement of the semester, students must advise the Department of Biochemistry and Molecular Biology of their order of preference for the alternative practical sessions and the other subjects they will be taking. (See subject website for details).

Biochemistry 521-211, 521-212 and 521-220.

BBiomedSc students: 521-213 and 536-250.

To participate in the rapidly expanding field of genome research, it is necessary to have an understanding of the techniques for handling both DNA and recombinant proteins. This subject aims to provide both. Students will receive training in the basic laboratory skills for manipulation of DNA and proteins and apply these skills to a number of biotechnological investigations.

Areas covered include the use of recombinant DNA for the investigation of gene function and the use of bacterial expression systems for the production and analysis of recombinant proteins. Specific experiments will deal with PCR, plasmid purification, DNA cloning and sequencing and bioinformatics.

In addition to these specific skills, students will develop an appreciation for the current scientific literature and acquire problem-solving skills through collaborative work.

Students will learn how to maintain a laboratory notebook containing a detailed record of the experiments carried out and prepare written reports describing these experiments.

The experimental work will be organised into elective streams, one of which will involve an opportunity to undertake relevant project work in one of the department's research laboratories (a quota will apply for project work).

The practical unit will be supported by a lecture series addressing current advances in these technologies. Topics include cDNA cloning and sequencing, sequence databases and analysis; recombinant expression systems; protein purification; and immunochemistry.

Ongoing assessment of laboratory skills and practical management of the experimental program throughout the semester (30%); a 2-hour written examination in the examination period (30%).

Laboratory course: a written research report of up to 2000 words and a laboratory notebook submitted during the semester (40%).

Project work: maintenance of a laboratory notebook throughout the semester and a written research report of up to 2500 words to be submitted by the end of the semester (40%).