521-302 Functional Genomics

Biochemistry 521-211, 521-212 and 521-220.

Other combinations that provide similar background will be considered by the coordinator.

BBiomedSc students: 521-213 and 536-250.

By the end of the subject, the student should have developed a detailed understanding of current concepts concerning the molecular bases of genome structure and the regulation of gene expression in eukaryotic organisms (yeast, animals and plants); a theoretical background to recombinant DNA technology and an appreciation of its biomedical and biotechnological applications; an appreciation of the significance and applications of human and related genome sequencing programs; and the ability to read critically original scientific literature in the field. Subject content includes structure of genes and chromosomes; identification and functional characterisation of candidate genes for human familial disease; molecular aspects of transcription and RNA maturation; regulation of gene expression at the transcriptional and translational levels; gene expression profiling and proteomics; ribosome biogenesis as a major example of the coordination of gene expression and RNA processing; biochemistry and molecular biology of cell cycle control and carcinogenesis; proto-oncogenes and tumour suppressor genes; ribozymes and the catalytic and antisense functions of RNA; applied genomics; and recombinant DNA technology, including recombinant protein expression systems with particular reference to investigations based on transfected cell culture, transgenic and gene knockout systems.

In addition to these specific skills, students will think critically from consideration of the lecture material and research papers, expand from theoretical principles to practical explanations through observing and reporting research literature, and acquire abilities in collaborative working while participating in group presentations.

A 15-minute group oral presentation or a 1500 word written assignment during the semester (10%); two 45-minute multiple choice examinations during the semester (5% each); a 3-hour written examination in the examination period (80%).

• B Alberts et al, Molecular Biology of the Cell. 4th edn, Garland, 2001.
  or
  H Lodish et al, Molecular Cell Biology. 4th edn, Scientific American Books, 2000.