411-335 Biochemical/Environmental Engineering 1B

411-334 Biochemical/Environmental Engineering 1A or equivalent science subject

Students successfully completing the course should have developed an understanding of the kinetics of enzyme and microbial processes and the factors affecting the design and operation of fermentation equipment.

Content: Enzymic processes. Michaelis-Menten approach. Kinetics of enzyme inhibition. Immobilised enzymes. Batch microbial growth and product formation. Continuous culture. Microbial growth kinetics. Application of Monod model to batch and chemostat culture. Kinetics of product formation. Maintenance energy and endogenous respiration. Design of fermentation processes. Medium formulation and inoculum preparation. Industrial sterilisation processes. Calculation of sterility level. HTST sterilisation. Design of continuous sterilisers. Air sterilisation. Vessel design for aseptic operation. Fermenter design configurations. Aeration of ermenters. Oxygen requirements of microorganisms. Mixing in fermenters. Biochemical separation processes.

One written 2-hour end-of-semester examination (100%).