531-302 Techniques for Investigation of Disease

Students intending to complete a major in pathology are required to enrol in both 531-301 and 531-302.

To enrol in 531-303 and 531-304, a pass must be achieved in both 531-301 and 531-302. Credit cannot be gained for 531-302 and 531-301 prior to 2000.

Basic Principles of Pathology 531-201, biochemistry and molecular biology 521-211/221 and 521-212/222. The following subjects are recommended: anatomy and cell biology 516-201; biochemistry and molecular biology 521-301 and/or 521-302; or microbiology and immunology 526-304 plus 526-324.

531-301 Cellular Basis of Disease.

Students completing this subject will:

• gain a deeper theoretical and practical understanding of the way in which questions about disease processes are formulated and investigation of these questions is carried out using sophisticated laboratory-based techniques; and

• take part in hands-on laboratory experiments using current techniques (see below) appropriate for investigation of a variety of diseases; also complete written exercises based on interpretation of unseen experimental data.

The techniques to be studied are immunofluorescence and immunocytochemistry, ELISA, immunoblotting and molecular biology techniques.

Practical laboratory work (continuous assessment) including work records and practical reports.

A laboratory manual and references to current scientific journal articles will be available at the beginning of the semester.