521-321 Gene Technology & Protein Expression

Before the commencement of the semester, students must advise the Department of Biochemistry and Molecular Biology of their order of preference for the alternative practical sessions and the other subjects they will be taking.

Biochemistry 521-211, 521-212 and either 521-220 or 521-221; or 521-024.

BBiomedSc students: 521-213 and 536-250.

To participate in the rapidly expanding field of genome research it is necessary to have an understanding of the techniques for handling both DNA and recombinant proteins. This subject aims to provide both. Students will receive training in the basis laboratory skills for manipulation of DNA and proteins and apply these skills to a number of biotechnological investigations.

In addition to these specific skills, students will develop problem-solving skills and acquire abilities in collaborative work.

The experimental work will be organised into elective streams, one of which will involve an opportunity to undertake relevant project work within one of the department's research laboratories (a quota will apply for project work). Areas covered include the use of recombinant DNA for the investigation of gene function and the use of bacterial expression systems for the production and analysis of recombinant proteins. Specific experiments will deal with nucleic acids, plasmid purification, DNA cloning and sequencing, and bioinformatics. The practical unit will be supported by a lecture series addressing current advances in these technologies. Topics include cDNA cloning and sequencing, sequence databases and analysis; recombinant expression systems; protein sequencing; and the use of antibodies.

Laboratory skills and practical management of the experimental program (30%); written research reports of up to 2000 words each (40%); written assignment of up to 2000 words or a 2-hour written examination (to be advised at the commencement of the subject) (30%).