521-321 Gene Technology & Protein Expression

Biochemistry 521-211, 521-212 and either 521-220 or 521-221; or 521-024.

BBiomedSc students: 521-213 and 536-250.

To participate in the rapidly expanding field of genome research it is necessary to have an understanding of the techniques for handling both DNA and recombinant proteins. This subject aims to provide both.

By the end of the subject students will have received training in the basic laboratory skills for manipulation of DNA and proteins and apply these skills to a number of biotechnological investigations. Particular areas covered will include the use of recombinant DNA for the investigation of gene function and the use of bacterial expression systems for the production and analysis of recombinant proteins. Specific experiments will deal with nucleic acids, plasmid purification, DNA cloning and sequencing and the use of elementary bioinformatics for the storage, retrieval and analysis of nucleotide sequence data. The subject will also include the production and analysis of proteins and enzymes created in a range of recombinant expression systems. The practical unit will be supported by a lecture series addressing current advances in these technologies. Topics may include cDNA cloning and sequencing, sequence databases and analysis; yeast and relate genomes and genome resources; recombinant expression systems; protein sequencing; western blotting; biological uses of radioisotopes.

The experimental work will be organised into elective streams, one of which will involve an opportunity to undertake relevant project work within one of the department's research laboratories (a quota will apply for project work).

Three components will be assessed: 1. laboratory skills and practical management of the experimental program; 2. written research report(s); 3. a written assignment of up to 2000 words or a 2-hour written examination (to be advised at the commencement of the subject).