516-301 Practical Cell Biology

Note

Experiments involving the use of animals are an essential part of this subject; exemption is not possible.

Credit Points

Coordinator

Prerequisites

Anatomy and Cell Biology 516-201; Cell Biology 606-206 or Cell Biology 606-216 or Biochemistry 521-211, 521-212 (Prior to 1997: 521-201)

Semester

Contact

Subject Description

Upon completion of this subject students should:

• comprehend the theoretical foundations of a range of techniques used in studying cell biology
• understand the type of information about cell biology that can be obtained using particular techniques
• appreciate the advantage of a thorough knowledge of the theory of a technique in order to fully understand the cell biological data acquired using that technique
• the strengths and limitations of techniques used to study cell biology

In the practical component students should:

• develop basic skills in performing techniques used to study cell biology

The theoretical foundations of the following techniques are considered: light microscopy, histology, transmission electron microscopy, scanning electron microscopy, immunohistochemistry, histochemistry, autoradiography, phase contrast microscopy, fluorescence microscopy, confocal microscopy, cell and tissue culture, morphometry, stereology, affinity chromatography, gel electrophoresis, and in situ hybridisation histochemistry.

Assessment

An essay during the semester, 2-hour end-of-semester written examination, practical work during the semester, and a 30-minute end-of-semester practical examination.