526-324 Immunological Techniques (Practical)

Credit Points:

Coordinator:

Corequisite/s:

Timetable:

Contact:

Objectives:

By the end of the subject students should:

Have developed:

• skills in the in vitro manipulation and quantitation of cells belonging to the immune system;

• skills in the measurement of cell function;

• skills in separation, detection and quantitation of immuno-globulins and antigens;

• an understanding of the basis of the serological diagnosis of disease.

Appreciate:

• the necessity to keep clear laboratory notes as experiments progress;

• the necessity for careful controls in interpretation of experiments.

Understand:

• the experimental basis of our knowledge of the immune response.

Content:

Preparation, characterisation, separation and assay of lymphocyte populations. Characterisation, separation and assay of antibodies and immunoglobulins. Assay of the immune response during infection. Detection of normal and abnormal antigens in tissues.

Assessment: