521-222 Biochemistry and Molecular Biology Laboratory: Part B

Note:

1. Students must advise the Department of Biochemistry and Molecular Biology of their order of preference for the following practical class times; Monday 2.00-5.00 pm, Tuesday 10.00-1.00 pm and Tuesday 2.00-5.00 pm (a fourth class (Friday 2.00-5.00pm) will be held if necessary), and the other subjects they will be taking. This should be done in person in the second last week of Semester One. Final allocation of students to each practical session will be posted in the Department by 10.00 am on the first Monday of Semester Two.

2. This subject should be taken by all students contemplating a full major in Biochemistry at 300 level. Restricted choices at 300 level apply to candidates presenting with only Part A of this practical course.

Credit Points:

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Objectives:

By the end of the program the student will have advanced manipulative skills and will have furthered the appreciation of practical procedures in biochemistry and molecular biology as stated for the prerequisite subject 521-221. Additional objectives will be to develop skills in the analyses of nucleic acids and in the use of computers for relevant experimental simulation and nucleic acid data base searching and analysis.

Content:

Experiments involving common techniques used in the purification and analysis of DNA and small molecules of biochemical and clinical importance; experimental and computer-based gene cloning and sequencing introduction to the use of computer-based DNA and protein data bases; designing application of enzyme assays; metabolic investigations; discussion of scientific literature. An opportunity is available for students to make scheduled visits to research laboratories within the Department. A limited and conditional exemption from experiments that use animals may be granted in some circumstances by the Head of the Department.

Assessment: