791-328 Analytical Techniques II

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Objectives:

The objective of this subject is to develop students' ability to:

• describe the physical, chemical and microbiological principles which underlie rapid and instrumental techniques for testing and analysing raw materials and finished products;

• evaluate innovative instrumental methods for specific purposes and materials against criteria of reliability and validity of results, and of cost and efficiency of monetary and labour resources;

• select rapid or instrumental methods for analyses appropriate to the type of evaluation or assessment required.

Content:

Each of the following types of analytical techniques will be studied in line with The objectives outlined: Physical, chemical, and microbiological parameters to be assessed; Principles of instrumentation and/or methodology and applications of these principles to the technologies employed in analytical techniques; Comparison of instrumental and/or rapid methods to conventional techniques of analysis; Operation, calibration and standardisation procedures as applicable to particular techniques; Assessment and evaluation of data derived from instrumental and/or rapid methods. Reliability, validity, cost, efficiency. Methods to be examined: Chromatographic, TLC, HPLC, GLC; Ion exchange separations; Spectrophotometry, UV, visible, AA; Mass spectrometry; Serological techniques, FA, ELISA, monoclonal antibody; DNA and RNA technology, probes, PCR; Electrophoretic separations; Impedance. Industrial and research applications.

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