Handbook 1996 : Faculty of Science (Volume 4 page 168)
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516-301 Practical Cell Biology

Note: Experiments involving the use of animals are an essential part of this subject; exemption is not possible.

Credit points: 16.0

Coordinator: Dr John F Bertram

Prerequisite: Anatomy and Cell Biology 516-201; 606-206/216 Cell Biology II or 521-201 Principles of Biochemistry and Molecular Biology in second year recommended.

Contact: 26 lectures (two a week) and 39 hours practical work

Timetable: First semester

Objectives:

This subject aims to provide a theoretical background and practical experience in a variety of techniques commonly used in studying cell biology. Upon completion of this subject students should:

• Comprehend the theoretical foundations of a range of techniques used in studying cell biology; the information about cell biology that can be obtained using particular techniques;
• Develop skills in performing techniques used to study cell biology; communication skills to describe the theoretical foundations and practical requirements of methods used to study cell biology; an appreciation of the scientific and methodological rigour needed to study cell biology;
• Appreciate the advantage of a thorough knowledge of the theory of a technique in order to fully understand the cell biological data acquired using that technique; the strengths and limitations of techniques used to study cell biology; the scientific basis of the knowledge of cell biology; the importance of oneí s own observations.

Content:

Light microscopy: tissue processing for light microscopy, sectioning and staining, immunohistochemistry, autoradiography, fluorescence microscopy, phase contrast microscopy, photographic techniques. Electron microscopy: tissue processing for electron microscopy, transmission and scanning electron microscopy. Cell and tissue culture. Quantitative morphology: morphometry, stereology.

Assessment:

A 2-hour end-of-semester written examination, practical work during the semester, and a 30-minute practical examination in the last week of the semester

Anatomy & Cell Biology subject : Next:516-302 | Prev:606-216 | Search | Help
Handbook 1996 : Faculty of Science (Volume 4 page 168)

Status:          Official 1996
Date created:    Oct  9 1995
Last modified:   Oct  9 1995
Authorised by:   Academic Registrar
Email enquiries: Course_Information@registrar.unimelb.edu.au

Copyright © University of Melbourne 1995,1996.