10.0 Professor W H Sawyer Biochemistry and Molecular Biology 521-201 and 521-202, or Biological Chemistry 521-024 First semester 26 lectures (two a week) By the end of the program the student should have acquired:

• An understanding of the relationships between molecular design, cellular organisation and biological function, as pertaining to eukaryotic cells and their subcellular organelles and biomembranes;
• A theoretical background to the major techniques used in the investigation of cytoskeletal structure, membrane lipid dynamics and membrane transport and associated energy-transducing processes.

Cytoskeletal components including actin assembly and disassembly, actin binding proteins, myosin, tubulin, intermediate filaments, clathrin. How cells move and change shape. Lipid dynamics and concepts of order and fluidity, lateral and rotational diffusion of lipids and protein. Collagen structure and biosynthesis; components of the extracellular matrix. Compartmentalisation in eukaryotic cells, intracellular RNA and protein traffic, chaperones; subcellular organelles, their biogenesis and function; protein folding and maturation; receptors and signal transduction. Energy transducing membranes and modern concepts of electron transport and oxidative phosphorylation; ion pumps and their regulation. A 2-hour end-of-semester written examination. Alberts B et. al. Molecular Biology of the Cell 3rd edition 1994 Garland 8 Prof W H Sawyer. 521-024 Biological Chemistry First semester 26 hours of lectures.